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1. To Study Of Commercially Available Probiotics And Prebiotics On The Performance And Nutrient Availablity In Japanese Quail

by Agha Shehzad Shaukst | Dr.Saima | Dr. Makhdoom Abdul Jabbar | Prof.Dr.Muham.

Material type: book Book; Format: print Publisher: 2009Dissertation note: Poultry feed accounts for 60 to 70% of total cost of production. To increase the efficiency of feed, different types of growth promoter likes enzymes and antibiotics have been used. But long term use of these antibiotics has side effects like antibiotic resistance and drug residues in meat. Many countries are regulating the use of antibiotics in feed and setting up programs to reduce the overall use of antibiotics. The impending ban on antibiotics in animal feed, the current concern over the spread of antibiotic resistance genes, the failure to identify new antibiotics make a compelling cause for developing alternative prophylactics. The use of probiotics and prebiotics is recommended as an alternative to antibiotics as prophylactic, therapeutic and growth-promoting agents in poultry production. Keeping this thing in view a 28 day long feeding trial was planned. A total of 420 day old Japanese quail chicks were randomly assigned to 7 dietary treatments each having 3 replicates of 20 birds. Seven diets were formulated in such a way that they contained similar levels of CP and ME (24% & 2900 kcal/kg) but different in Probiotics and Prebiotics levels. Diet A was ,in accordance with the nutritional requirements of Japanese quail as specified in NRC (1994) whereas, diets B, C and D contained probiotics 50mg, 75mg and 100mg respectively. Diets E, F and G contained Prebiotics ig, 2g and 3g respectively. Feed consumption, body weight gain, FCR, dressing percentage, giblet weight, plasma calcium magnesium and phosphorus, zinc retention, plasma cholesterol, economics of all experimental rations were the parameters considered. Data thus obtained were subjected to statistical analysis using Analysis of Variance (ANOVA) Technique. The difference among the treatment means were tested by Duncan's Multiple Range (DMR) Test. The results of the experiment revealed that body weight gain, feed consumption, FCR, were significantly (p<O.O5) higher in probiotic and prebiotic added groups. Whereas plasma calcium was significantly (p<O.O5) higher in brebiotics added groups than control and prohiotics added groups. Plasma cholesterol was significantly lower in probiotic added groups. Plasma magnesium, phosphorus, zinc concentration, giblet weight and dressing percentage showed nonsignificant (p>O.O5) difference among all the groups. Data pertaining to economics of the experiment revealed a considerably low cost of feed/kg of the group B and C. While the group D and prebiotic added groups did not decreased the feed cost. Conclusion: o Use of probiotics & prebiotics increased the weight gain but the effect of prohiolics was more pronounced o Prebiotics increased the Ca absorption o Probiotics decreased the plasma cholesterol o Use of probiotics proved to be economical although prebiotics also improved the weight gain and FCR but it is not economical to use it as growth promoter From the results of above experiment it is concluded that inclusion of prehiotics have no considerable benefits but the use of probiotics at low concentration is useful. This consequently results in considerably improved performance and ultimately higher profitability of the farm. Availability: Items available for loan: UVAS Library [Call number: 1101,T] (1).

2. Effect Of Form Of Feed And Dietary Supplementation Of Phytase And Vitamin D3 On Phytase Utilization In Broilers

by Muhammad Farooq | Dr.Saima | Dr. Makhdoom Abdul Jabbar | Dr.Farina Malik.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 2008Dissertation note: AThe purpose of this research trial was to study the effect of form of feed and dietary supplementation of phytase and vitamin 1)3 on phytate utihzation. growth performance, minerals (calcium and phosphorus) and nitrogen retention in broilers. I he findings of this study on the above mentioned parameters have been discussed as under in the light of the available literature. One hundred and eighty day-old broiler chicks were purchased from local market. The chicks were weighed on the first day and then randomly divided into 6 groups of 18 replicates. [here were ten chicks in each replicate. Six isocaloric (M[ 2750 Real/kg) and isonitroginous (CP% 19) broiler starter rations (A, B, C, 1), E and F) were formulated. Similarly six isocaloric (ME 2850 kcal/kg) and isonitroginous (CP% 18) broiler finisher rations (A, B, C, D, E and F) were formulated. Ration A and ration B was control but ration A was in mash form and ration B in the Form of pellets. Rations C and I) were Formulated in mash form with phytase (Phyzyme XP 10000 LV!) 500 FlU/Kg along with 1, 25(OH) 2 1)3 a' 2000 and 3000 lU/Kg respectively. Rations E and F were formulated in pellets form with phytase (Phyzyme XP 10000 TPT) @ 500 FTU/Kg along with 2000 and 3000 lU/Kg of 1, 25(OH) 2 D3 respectively. Starter diets were fed I to day 28 and finisher diets were led from day 29 th to 42 Ui day. All the birds were provided ICed and water ud-/ibiluin and were provided with 24 hours light. The parameters defined for study were feed intake, weight, FCR, dressing percentage, toe ash percentage, calcium and phosphorus in toe ash and excreta, nitrogen retention and economics. The data was subjected to statistical analysis and differences among treatment means were analyzed through l)MRl lest. The results indicated average feed intake that highest feed intake was found in group F (D3 3000 lU/kg and phytase 500 units/kg) and E (1)3 2000 lU/kg and phytase 500 units/kg,) which were in pellet form respectively. The statistical analysis showed significant difference (P<0.05) in feed intake of the birds fed diets in pellet and mash form containing varying levels of D3 and phytase level. The lowest feed intake was found in group A (control, 0 phytase and 0 D3 level) which was in mash form. The maximum average weight gain was in the birds fed diets containing phytase 500 units/kg and vitamin D3 containing 2000 and 3000 units/kg in group F and F in pellet form respectively, while minimum weight gain was in the birds fed diets containing no phytase and vitamin D3 ( group A in mash form). The statistical analysis showed significant difference (P<0.05) in weight gain of the birds fed diets containing varying levels of phosphorus. 1)3 and phytase (a) 500 units with pellet and mash form. Ihe poor FCR was found in group F and best ICR was observed in group E. The statistical analysis showed non-significant difference (P<0.05) in feed conversion ratio of the birds of all the groups. The highest dressing percentage was observed in group F (phytase S00units/kg and vitamin D3, 3000 units/kg) in pellet form followed by group 13 (control. pellet) and F (phytase S00units/kg and vitamin 1)3, 2000 units/kg). The lowest dressing percentage was observed group A (control, mash).A significant difference was found between dressing percentage of all the groups (P<0.05). Statistical analysis showed non-significant difference between all the groups (P<0.05). Highest toe ash percentage was observed in group D (phytase So0units/kg and vitamin D3, 3000 units/kg) in mash form) followed by group A. C and F. The lowest toe ash percentage was found in group F. The statistical analysis showed non-significant difference among all the groups (P<0.05). The highest phosphorus in toe bone was in group D followed by F and F and lowest phosphorus was found in group A. The results showed that non-significant difference was found between all groups (P<O.05). The highest calcium in toe bone was in group A and F and lowest calcium was in group D. There was non-significant difference between all the groups (P<O.05). Calcium excretion in group B was lowest while calcium excretion in group A was highest. The statistical analysis showed that there was non-significant difference between all the groups (P<O.05). The lowest phosphorus excretion was found in group F and highest phosphorus excreted in group B and E. The highest nitrogen retention was observed in group F. There was significant difference between all the groups (P<O.05). A significant difference was found in all the groups at 6th week (P<O.05). The highest nitrogen retention was observed in group E and F. Net profit was highest in F and F followed by B and D. Availability: Items available for loan: UVAS Library [Call number: 1103,T] (1).

3. Bioavailability Of Macro Minerals (Ca,P,Na,K)In Different Feed Stuffs Commonly Used In Broilers Ration

by Arslan Farooq | Prof.Dr.Talat Naseer Pasha | Dr.Farina Malik | Dr.Saima.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2009Dissertation note: Many reports have illustrated the limitations of feed stuffs in meeting the mineral needs of poultry and other monogastric species. Many feed millers base their estimates of requirements on the NRC. However, the commercial feed industry has adjusted these levels based on their own 'gut feel' rather than on any scientific basis. So, when poultry feed is formulated on this basis, it may not fulfill the bird's nutritional requirement due to their tabulated values instead of actual, resulting in some nutritional disorder and many other related problems. Therefore it becomes more imperative that we must have nutrient profile of our local feed stuff. The present study has been designed to ascertain mineral profile of the poultry feedstuffs (cotton seed meal, wheat bran, corn gluten meal 60% and corn gluten meal 30%) locally available to the industry and prepare a document on the basis of data analyzed which will be used as a ready reference through out the country. Evaluation of the minerals (Ca, Na, K, P) bioavailability of these ingredients and commercial broiler starter feed in broiler was also be determined. For this purpose poultry feed ingredients and commercial broiler starter feed were procured from a commercial feed mill. For bioavailability of minerals a total of 200 day old Hubbard broiler chicks were used and allocated to five (5) dietary treatments (A, B, C, D and E) in such a way that each treatment was replicated 4 times with 10 chicks in each replicate. Diet A was of corn- soybean meal. Diet B, C, D and E was contain cotton seed meal, wheat bran, corn gluten meal 60% and corn gluten meal 30 %, respectively. The data obtained was subjected to statistical analysis using Analysis of Variance Technique. The difference among the treatment means was tested through Duncans's Multiple Range (DMR) Test. The maximum Ca bioavailability was observed in group B (61.56±5.51%) which was commercial feed, followed by group C (46.30 ±4.29) containing cotton seed meal, E (41.61±8.05) containing corn gluten meal 60%, A (37.60±5.0) containing control(corn- soybean meal), D (35.12±9.20) which was wheat bran and F (22.3 1±8.04) containing corn gluten meal30%. There was a significant (P<0.05) difference in bioavailability of Ca in group A between the groups B and F, respectively. The maximum P bioavailability was observed in group A (58.48± 3.59) which was control feed, followed by group D (52.32±3.09) containing wheat bran, B (49.06±5.81) containing control feed, E (45.94±7.12) containing corn gluten meal 60%, C (40.45±4.55) which was cotton seed meal and F (38.68±8.3 1) containing corn gluten meal 30%. There was a significant (P<0.05) difference in bioavailability of phosphorus in group A among the group B C, E and F, respectively. The maximum Na bioavailability was observed in group B (77.50± 5.86) which was commercial feed, followed by group C (75.03±3.28) containing cotton seed meal, F (73.62±4.75) containing corn gluten meal 30% feed, D (73.10±4.5 1) containing wheat bran, A (65.05±7.16) which was control diet and E (58.40±7.34) containing corn gluten meal 60% respectively. There was a significant (P<0.05) difference in bioavailability of Na in group A among the groups B, C, D, E and F respectively. The maximum K bioavailability was observed in group A (94.17± 0.85) which was control feed, followed by group B (93.98±0.51) containing commercial feed, E (93.05±1.05) containing corn gluten meal 60% feed, D (91.62±0.84) containing wheat bran, F (90.69±0.69) which was corn gluten meal 60% feed and C (90.26±1.07) containing cotton seed meal respectively. There was a significant (P<0.05) difference in bioavailability of K in group A among the groups C, D, E and F, respectively. Availability: Items available for loan: UVAS Library [Call number: 1106,T] (1).

4. Efficacy Of Commercially Available Natural Enzyme Complex On Performance And Nutrient Availability In Broiler Rations

by Asim Munawar | Prof.Dr.Talat Naseer Pasha | Dr.Saima | Prof.Dr.Muhammad Akram.

Material type: book Book; Format: print Publisher: 2003Dissertation note: Poultry feed is mainly composed of cereals and vegetable proteins, which cannot be fully digested and utilized by poultry due to lack of endogenous enzymes. As feed costs 70% of total poultry operation, so there is need reconsider enzyme supplementation in such feeds. For this purpose, a total of 800 day old Hubbard broiler chicks will be randomly selected, weighed, and assigned to 5 dietary treatments with 8 replicates per treatment. Feed was procured from a commercial feed mill, comprising of a control diet (2750 Kcal/kg energy with no Allzyme SSF), two negative control diets (Negative Control Diet-I and II having 2700 Kcal/kg and 2675 Kcal/kg energy having no Allzyme SSF) and two positive control diets (2700 Kcal/kg and 2675 Kcal/kg energy having Allzyme SSF @ 200gms/ton). The average body weights of broilers at different ages showed that broilers of group A attained maximum weight, which was followed by group D, B, E and C, respectively, revealed that the weights of group B, C and E were significantly different from group A, whereas, the differences of group A and D were found non significant that maximum feed consumption was exhibited by group B followed by group C, E, D and A. The data of feed consumption when subjected to analysis of variance showed significant difference (p<0.05) of group B and C with groups A, D, E, while the feed consumption of group B and C was found insignificantly different from each other. The overall FCR of group A was found to be the best among all groups. Next to this was group D, followed by group E, B and C. It is evident from the results that FCR of broilers in group D was very near to control group A, showing activity of enzyme complex in terms of performance. The highest toe ash percentage (10.83%) was recorded in broilers of group D which was fed diet with 0.1% less available phosphorus and 0.1% calcium but added 200gm/ton enzyme complex, lowest toe ash percentage (9.98%) was recorded in group C which was fed with diet having 0.1% less available phosphorus and 0.1% calcium than control group A with no enzyme complex supplementation. The highest Ca content in plasma was observed in group A, whereas the lowest Ca plasma content was recorded in group B. Phosphorous content in plasma of group D was found to be highest among all the groups followed by E, A, B and C. No bird had showed any kind of leg weakness/deformity. Critical analysis of the data indicated that birds of group D were reared at lowest feed cost and this diet was found most economical. Cost of production of feed and per kg live weight can be decreased by use of enzyme complex in feed. Availability: Items available for loan: UVAS Library [Call number: 1286,T] (1).



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